In Vitro Propagation of Cremastra appendiculata var. variabilis by Asymbiotic Seed Germination

Cremastra appendiculata var. variabilis (Blume) I.D. Lund), also known as single-leaf cremastra (SLC), is a rare and threatened species native to Korea, it has the potential be grown beautiful flowering pot or garden plant. There still no reliable strategy multiply SLC. Thus, an effective method for propagating was needed its conservation mass production. In present study, we examined effects of culture media, seed age, activated charcoal, plant growth regulators on in vitro asymbiotic germination, secondary protocorm induction, seedling formation. Asymbiotic germination SLC influenced by their interaction. The addition charcoal (500 mg/L) Murashige Skoog (MS) medium increased rate germination. seeds were best germinated (91.9%) culturing MS supplemented with mg/L), α-naphthaleneacetic acid (3 µM), kinetin (1 µM). highest number (28.9) protocorms produced when cultured containing 6-benzyladenine (4 µM) (2 When cultivated gibberellic they able transform into success (78.7%). propagation protocol described here may helpful restoration programs large-scale